human il 6 elisa kit  (R&D Systems)

Journal: Central-European Journal of Immunology

Article Title: p65 enhanced METTL3-mediated m 6 A methylation of HMGB1 to promote microglia M1 polarization in sepsis-associated encephalopathy

doi: 10.5114/ceji.2025.154882

Figure Lengend Snippet: p65, METTL3, and HMGB1 increased in lipopolysaccharide (LPS)-induced HMC3 cells. HMC3 cells were stimulated with LPS. A ) CCK-8 kit was assessed using cell viability. B ) The proportion of CD86+CD11b+ cells in HMC3 cells was evaluated using flow cytometry. Data are presented as means ± SD. Significance levels were: * p < 0.05, ** p < 0.01, and *** p < 0.001 C ) Inflammatory cytokines including TNF-α, IL-1β, and IL-6 were measured by ELISA kits. D ) Western blot was performed to detect the levels of iNOS, p-p65, METTL3, and HMGB1. All experiments were performed in triplicate. Data are presented as means ± SD. Significance levels were: * p < 0.05, ** p < 0.01, and *** p < 0.001

Article Snippet: Concentrations of inflammatory cytokines, such as tumor necrosis factor α (TNF-α, #DTA00D), interleukin 1β (IL-1β, #DLB50), and IL-6 (#D6050), were assessed using ELISA kits from R&D Systems located in Minneapolis, MN, USA.

Techniques: CCK-8 Assay, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: Central-European Journal of Immunology

Article Title: p65 enhanced METTL3-mediated m 6 A methylation of HMGB1 to promote microglia M1 polarization in sepsis-associated encephalopathy

doi: 10.5114/ceji.2025.154882

Figure Lengend Snippet: p65 activated METTL3 promoted LPS-induced microglia M1 polarization HMC3 cells were transfected with sh-METTL3 and/or oe-p65 and stimulated with LPS. A ) qRT-PCR was used to detect the expression of p65 and METTL3. B ) A CCK-8 kit was used to assess cell viability. C ) Flow cytometry was used to evaluate the proportion of CD86+CD11b+ cells among HMC3 cells. Data are presented as means ± SD. Significance levels were considered as follows: * p < 0.05, ** p < 0.01, and *** p < 0.001 C ) Flow cytometry was used to evaluate the proportion of CD86+CD11b+ cells among HMC3 cells. Data are presented as means ± SD. Significance levels were considered as follows: * p < 0.05, ** p < 0.01, and *** p < 0.001 D ) TNF-α, IL-1β, and IL-6 levels were measured by ELISA kits. E ) Western blot was performed to detect iNOS, p-p65, and METTL3 expression. All experiments were performed in triplicate. Data are presented as means ± SD. Significance levels were considered as follows: * p < 0.05, ** p < 0.01, and *** p < 0.001

Article Snippet: Concentrations of inflammatory cytokines, such as tumor necrosis factor α (TNF-α, #DTA00D), interleukin 1β (IL-1β, #DLB50), and IL-6 (#D6050), were assessed using ELISA kits from R&D Systems located in Minneapolis, MN, USA.

Techniques: Transfection, Quantitative RT-PCR, Expressing, CCK-8 Assay, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: Central-European Journal of Immunology

Article Title: p65 enhanced METTL3-mediated m 6 A methylation of HMGB1 to promote microglia M1 polarization in sepsis-associated encephalopathy

doi: 10.5114/ceji.2025.154882

Figure Lengend Snippet: METTL3 up-regulated HMGB1 and enhanced LPS-induced microglia M1 polarization. HMC3 cells were transfected with sh-HMGB1 and/or oe-METTL3 and stimulated with LPS. A ) qRT-PCR was used to detect the expression of METTL3 and HMGB1. B ) A CCK-8 kit was used to assess cell viability. C ) Flow cytometry was used to evaluate the proportion of CD86+CD11b+ cells among HMC3 cells. Data are presented as means ± SD. Significance levels were considered as follows: * p < 0.05, ** p < 0.01, and *** p < 0.001 C ) Flow cytometry was used to evaluate the proportion of CD86+CD11b+ cells among HMC3 cells. Data are presented as means ± SD. Significance levels were considered as follows: * p < 0.05, ** p < 0.01, and *** p < 0.001 D ) TNF-α, IL-1β, and IL-6 levels were measured by ELISA kits. E ) Western blot was performed to detect iNOS, HMGB1, and METTL3 expression. All experiments were performed in triplicate. Data are presented as means ± SD. Significance levels were considered as follows: * p < 0.05, ** p < 0.01, and *** p < 0.001

Article Snippet: Concentrations of inflammatory cytokines, such as tumor necrosis factor α (TNF-α, #DTA00D), interleukin 1β (IL-1β, #DLB50), and IL-6 (#D6050), were assessed using ELISA kits from R&D Systems located in Minneapolis, MN, USA.

Techniques: Transfection, Quantitative RT-PCR, Expressing, CCK-8 Assay, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Western Blot